Research

I am a young researcher and academic staff of Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada. Biochemistry and Molecular Biology Laboratory is the laboratory where i work on. The laboratory conducts several laboratory works for undergraduate students i.e. DNA plasmid isolation, transformation plasmid DNA into bacteria, isolation of human DNA, genetic polymorphism of metabolic gene (CYP1A1, GSTM1, and CYP2D6), bioinformatics (analyzing probe, protein homology and conserved domain analyses), protein purification and protein sequencing (dry lab).

I joined into one of the Faculty of Pharmacy UGM research groups named Cancer Chemoprevention Research Center (CCRC).  CCRC has plenty collaborative researches with other institutions (universities, other research groups, industries, government research institutions). I am active contributing in CCRC, supervising the undergraduate students’ research and doing my research projects with the other CCRC members.

The in vitro researches included observing cytotoxicity of Indonesian plant’s extract and curcumin analogues in several cancer cells e.g.  breast cancer cell line (T47D; MCF-7), cervical cancer cell line (HeLa), colon cancer (WiDr), macrophage cell line (RAW 246.7), myeloma cancer cell line, Vero cell line, NIH 3T3 cell line, human embrionic kidney (HEK 293) cell line and EAHY cell line.

Besides cytotoxicity, CCRC also investigate the chemopreventive and/or antiproliferative effect of sample using double staining, immunocytochemistry and AgNOR staining, flowcytometry and western blot. CCRC’s works in vivo i.e. studying chemopreventive effect in rat induced by 7,12-dimethylbenz[a]anthracene (DMBA) or benzo(a)pirene (BAP); investigating antiangiogenesis effect in chick chorioallantoic membrane. CCRC also conducted research using ovariectomized rats in order to explore phytoestrogen from natural products. Observations are done by using Haematoxylin-Eosin, TUNEL assay, AgNOR and immunohistochemistry staining. CCRC also developed bioinformatics research using Basic Local Alignment Search Tool (BLAST) and study the docking of a compound and receptor using some software such as PLANTS and MOE.

My topic for PhD is exploring mechanism chemoresistance of cancer cells toward novel drug in genetic and proteomics level, and also develop therapeutic strategies to overcome chemoresistance.

Please feel free to contact me regarding to my research topic and research collaboration

Vielen dank!!